HOF/NIL Phenotype SOP

HOF/NIL 2015 Complete Phenotype SOP

REMINDER: As soon as reasonable, low density plots should be thinned to 8 plants per plot.

For traits that are measured on individual plants, we will be measuring 6 plants per plot. For the low density plots this will be plants 2-7 in the plot and for the high density plots this will be plants 5-10.

If there are less than 8 plants in the low density plots the number of plants that are measured will be the number of middle plants (i.e. if there are 6 plants 4 will be measured).

Requested traits on all plots

• Plant height after flowering - base of the plant to the ligule of the flag leaf measured to the nearest cm
• Flowering time on a per plot basis - days between planting and 50% of plants of a plot exhibiting anther exertion on more than half of the main tassel spikes (anthesis) and days between planting and 50% of plants of a plot showing silk emergence (silk)
• Ear height - base of the plant to the primary ear bearing node to the nearest cm
• Ear and seed scans and image extraction with UW pipeline (ear length, ear width, kernel length, width, depth, etc.) measured on 6 plants per plot
• 100 kernel weight (plot measure in grams)

Additional optional traits that we will be measuring at UMN

• Weekly plant height before flowering - starting at ~V1 we will be measuring plant height every Wednesday. This will be done by pulling all leaves directly up and measuring to the top of the top leaf to the nearest cm.
• Leaf number - at V7 the 5th leaf will be punched, at V10 a collar will be placed between the 8th and 9th leaf, leaf number at maturity will be measured using the collar as a reference point.
• Ear weight (6 ears from individual plants in grams)
• Cob weight (6 cobs from individual plants in grams)
• Leaf number above and below the ear
• (?) Possibly measuring tassel branch number and angel

Mathematically determined traits

• Average kernel number (ear weight - cob weight / kernel weight from 100 kernel weight)
• Average internode length, average internode length above the ear, average internode length below the ear (plant height / leaf number; plant height - ear height / number leaves above the ear; ear height / number leaves below the ear)