Ear Hand Measurement and Scanning SOP

We have uploaded the SOP for the ear hand measurements and scanning for the HOF/NIL experiment located at this link. There is also a template decoding file and example decoding file for reference. Please email me at cnhirsch@umn.edu or Jacob Garfin at garf0012@umn.edu with any questions about the ear SOP.

UMN location 1 planting 2 flowering time data is here.

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UMN location 1 planting 1 flowering time data is here.

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UMN location 1 planting 2 inbred plant height measured on 7/22/15. File with data is here. Plants were V12-V13. This is the last plant height measurement at this location.

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UMN location 1 planting 1 and 2 plant height measured on 7/15/15. Files with data are here.

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UMN location 1 planting 2 collars were put on between the 10th and 11th leaves in the inbred plots on 07/13/15. 

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UMN location 1 planting 1 and 2 plant height measured on 7/08/15. Files with data are here. The first planting was at ~V12 and the hybrids in this planting are too tall to measure after this time point.

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UMN location 1 planting 2 collars were put on between the 10th and 11th leaves in the hybrid plots on 07/08/15. The inbreds are not far enough along and will be collared later in the week.

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UMN location 1 planting 1 collars were put on between the 10th and 11th leaves on 07/01/15-07/02/15.

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Phenotyping Information:

UMN location 1 planting 1 and 2 plant height measured on 7/01/15. Files with data are here.

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UMN location 1 planting 2 5th leaves were punched on 06/22/15.

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Phenotyping Information:

UMN location 1 planting 1 and 2 plant height measured on 6/24/15. Files with data are here.

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Phenotyping Information:

UMN location 1 planting 1 and 2 seedling plant height measured on 6/17/15. Files with data are here.

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UMN location 1 planting 1 5th leaves were punched on 06/15/15.

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This post is in regards to Lisa's comment below. I couldn't figure out how to embed a figure in a comment, so this is a new post.

Below is a photo showing how we are collecting plant height. Essentially the leaves are all pulled up and the highest leaf tip from the soil surface is measured. In this case, we would record this plant as 23cm. Let me know if there are still questions.

Planting dates for ISU locations:

Location 1 Planting 1: 5/19/15
Location 1 Planting 2: 6/1/15

Location 2 Planting 1: 6/2/15
Location 2 Planting 2: 6/10/15

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Phenotyping Information:

UMN location 1 planting 1 and 2 seedling plant height measured on 6/10/15. Files with data are here.

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The UMN location 1 planting 2 low density plots were thinned from 12 to 8 plants on 6/10/15.

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The UMN location 1 (St. Paul) first and second plantings were sprayed with atrazine on 6/8/15.

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The UMN location 1 planting 1 low density plots were thinned from 12 to 8 plants on 6/5/15.

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Phenotyping Information:

UMN Location 1 Planting 1 seedling plant height measured on 6/3/15. File with data is here.

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Planting Dates for UMN locations:

Location 1 Planting 1 - 5/5/15
Location 1 Planting 2 - 5/20/15
Location 2 Planting 1 - 6/2/15

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HOF/NIL Phenotype SOP

HOF/NIL 2015 Complete Phenotype SOP

REMINDER: As soon as reasonable, low density plots should be thinned to 8 plants per plot.

For traits that are measured on individual plants, we will be measuring 6 plants per plot. For the low density plots this will be plants 2-7 in the plot and for the high density plots this will be plants 5-10.

If there are less than 8 plants in the low density plots the number of plants that are measured will be the number of middle plants (i.e. if there are 6 plants 4 will be measured).

Requested traits on all plots

• Plant height after flowering - base of the plant to the ligule of the flag leaf measured to the nearest cm
• Flowering time on a per plot basis - days between planting and 50% of plants of a plot exhibiting anther exertion on more than half of the main tassel spikes (anthesis) and days between planting and 50% of plants of a plot showing silk emergence (silk)
• Ear height - base of the plant to the primary ear bearing node to the nearest cm
• Ear and seed scans and image extraction with UW pipeline (ear length, ear width, kernel length, width, depth, etc.) measured on 6 plants per plot
• 100 kernel weight (plot measure in grams)

Additional optional traits that we will be measuring at UMN

• Weekly plant height before flowering - starting at ~V1 we will be measuring plant height every Wednesday. This will be done by pulling all leaves directly up and measuring to the top of the top leaf to the nearest cm.
• Leaf number - at V7 the 5th leaf will be punched, at V10 a collar will be placed between the 8th and 9th leaf, leaf number at maturity will be measured using the collar as a reference point.
• Ear weight (6 ears from individual plants in grams)
• Cob weight (6 cobs from individual plants in grams)
• Leaf number above and below the ear
• (?) Possibly measuring tassel branch number and angel

Mathematically determined traits

• Average kernel number (ear weight - cob weight / kernel weight from 100 kernel weight)
• Average internode length, average internode length above the ear, average internode length below the ear (plant height / leaf number; plant height - ear height / number leaves above the ear; ear height / number leaves below the ear)

Trait Measuring

Hi All:

I wanted to try out posting in the blog about my question regarding trait measuring.

Can something be posted about the following:

• At what developmental stage should traits be started to collect
• What traits should be collected
• How frequently should a trait be taken (once and done, weekly, every other week, monthly)
• How many plants per plot should have traits taken (avoid plants on ends of plots?)
• An SOP with regards to how to measure a trait so that we're all collecting information the same way.

I have some notes from a meeting, but I wanted to make sure I have it correctly down in notes and that we're all on the same page across locations.

SOP can wait a little, but I'm interested in knowing the others soon so that I can better plan out labor for the summer.

Thanks,
Lisa

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Seed was shipped to Iowa today. The FedEx tracking numbers are 773482654068 and 773482654620.

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The final experimental design file has been posted on google drive and can be accessed here.

There is not enough seed for some of the hybrid HOF diallel crosses. As such we have removed replicates 13, 14, 15, and 16 from the Minnesota location. Additionally for the following hybrids there will be fill (Mo17 x B73) for replicates 9 and 10 (MN) and 29, 30, 33, 34, 35, and 36 (IA).

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Thank you all for collaborating on this project. We have set up this blog to post any information about this experiment for the entire group to see. Additionally, we have a google drive folder that we will use to archive files such as experimental design, phenotype data, etc. You can access the google drive folder by clicking here. Currently the blog and google drive are set to all can view. Please provide a list of people from your group that will be uploading files so that they can be granted edit permissions.

Posts to the blog can be made a https://www.blogger.com and the actual blog feed will be viewable at http://hofnil.blogspot.com. Hash tags can be used to make searching posts easier in this blog. As such, we ask that any posts pertaining to Iowa contain a line at the end that is #IA_HOFNIL, anything for Wisconsin to contain #WI_HOFNIL, and for Minnesota to contain #MN_HOFNIL. Note this hash tag needs to be on a line of its own for searching. Additionally, the entire contents of the blog can be exported as a flat file at any time through https://www.blogger.com.

Please email cnhirsch@umn.edu if there are any questions about accessing the blog or the google drive.