UMN location 1 planting 2 5th leaves were punched on 06/22/15.

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Phenotyping Information:

UMN location 1 planting 1 and 2 plant height measured on 6/24/15. Files with data are here.

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Phenotyping Information:

UMN location 1 planting 1 and 2 seedling plant height measured on 6/17/15. Files with data are here.

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UMN location 1 planting 1 5th leaves were punched on 06/15/15.

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This post is in regards to Lisa's comment below. I couldn't figure out how to embed a figure in a comment, so this is a new post.

Below is a photo showing how we are collecting plant height. Essentially the leaves are all pulled up and the highest leaf tip from the soil surface is measured. In this case, we would record this plant as 23cm. Let me know if there are still questions.

Planting dates for ISU locations:

Location 1 Planting 1: 5/19/15
Location 1 Planting 2: 6/1/15

Location 2 Planting 1: 6/2/15
Location 2 Planting 2: 6/10/15

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Phenotyping Information:

UMN location 1 planting 1 and 2 seedling plant height measured on 6/10/15. Files with data are here.

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The UMN location 1 planting 2 low density plots were thinned from 12 to 8 plants on 6/10/15.

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The UMN location 1 (St. Paul) first and second plantings were sprayed with atrazine on 6/8/15.

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The UMN location 1 planting 1 low density plots were thinned from 12 to 8 plants on 6/5/15.

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Phenotyping Information:

UMN Location 1 Planting 1 seedling plant height measured on 6/3/15. File with data is here.

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Planting Dates for UMN locations:

Location 1 Planting 1 - 5/5/15
Location 1 Planting 2 - 5/20/15
Location 2 Planting 1 - 6/2/15

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HOF/NIL Phenotype SOP

HOF/NIL 2015 Complete Phenotype SOP

REMINDER: As soon as reasonable, low density plots should be thinned to 8 plants per plot.

For traits that are measured on individual plants, we will be measuring 6 plants per plot. For the low density plots this will be plants 2-7 in the plot and for the high density plots this will be plants 5-10.

If there are less than 8 plants in the low density plots the number of plants that are measured will be the number of middle plants (i.e. if there are 6 plants 4 will be measured).

Requested traits on all plots

• Plant height after flowering - base of the plant to the ligule of the flag leaf measured to the nearest cm
• Flowering time on a per plot basis - days between planting and 50% of plants of a plot exhibiting anther exertion on more than half of the main tassel spikes (anthesis) and days between planting and 50% of plants of a plot showing silk emergence (silk)
• Ear height - base of the plant to the primary ear bearing node to the nearest cm
• Ear and seed scans and image extraction with UW pipeline (ear length, ear width, kernel length, width, depth, etc.) measured on 6 plants per plot
• 100 kernel weight (plot measure in grams)

Additional optional traits that we will be measuring at UMN

• Weekly plant height before flowering - starting at ~V1 we will be measuring plant height every Wednesday. This will be done by pulling all leaves directly up and measuring to the top of the top leaf to the nearest cm.
• Leaf number - at V7 the 5th leaf will be punched, at V10 a collar will be placed between the 8th and 9th leaf, leaf number at maturity will be measured using the collar as a reference point.
• Ear weight (6 ears from individual plants in grams)
• Cob weight (6 cobs from individual plants in grams)
• Leaf number above and below the ear
• (?) Possibly measuring tassel branch number and angel

Mathematically determined traits

• Average kernel number (ear weight - cob weight / kernel weight from 100 kernel weight)
• Average internode length, average internode length above the ear, average internode length below the ear (plant height / leaf number; plant height - ear height / number leaves above the ear; ear height / number leaves below the ear)